The role of the ASD-associated 16p11.2 gene QPRT during differentiation of human embryonic stem cell-derived cerebral organoids
Clara Droell, Julia Schwarzpaul, Silvia Lindlar, Afsheen Kumar, Andreas G. Chiocchetti, Denise Haslinger · FENS Forum 2024
The autism spectrum disorder (ASD)-associated 16p11.2 gene quinolinate phosphoribosyltransferase (QPRT) is essential for neuronal differentiation but not proliferation of SH-SY5Y neuroblastoma cells. QPRT is part of tryptophan metabolism and catabolizes quinolinic acid (QUIN), a NMDA-R agonist neurotoxic for glutamatergic cells. However, we can exclude a QUIN-driven neurotoxicity in the SH-SY5Y model. In primary human neural progenitor cells, chemical inhibition of QPRT using phthalic acid (PA) leads to premature differentiation and a shift from neurons to astrocytes. Here, we employed cerebra
ePoster · Neuroscience