Glial cells undergo rapid changes following acute chemogenetic manipulation of a subpopulation of layer 5 projection neurons
Auguste Vadisiute, Elise Meijer, Rajeevan Therpurakal, Marissa Mueller, Florina Szabo, Fernando Messore, Alfonsas Jursenas, Lukas Krone, Edward Mann, Vladyslav Vyazovskiy, Anna Hoerder-Suabedissen, Zoltan Molnar · FENS Forum 2024
Communication between neurons and glial cells are crucial to establish and maintain brain function. Some of these interactions are activity-dependent, yet it remains unexplored how acute changes in neuronal activity affect neuron-to-glial dynamics.Here, we used excitatory and inhibitory designer receptors exclusively activated by designer drugs (DREADD) based chemogenetic methods to study the effects of acute manipulations of a subpopulation of layer 5 cortical projection and dentate gyrus neurons in adult (Rbp4Cre) mouse brain after 90 minutes. We validated changes in activity with in vitro p
ePoster · Neuroscience