Topic: REV-ERBα

Our goal was to establish in vitro models using minipig brain cells to Establish the Cell Lines Detecting Circadian Rhythm Gene Expression. We isolated various brain cell types and generated continuously proliferative cell lines from primary cultured cells. These cell lines were extensively characterized using relevant markers to ensure suitability for studying circadian rhythm-related genes. Through CRISPR-Cas9 technology, we engineered cell lines expressing key circadian rhythm-related genes like PER, REV-ERBα, and HTR1A, confirmed by red fluorescent markers. Subsequent RNA sequencing analys