Protein-lipid binding properties implicate Piccolino in synaptic vesicle tethering at photoreceptor ribbon synapses
Michalina Gadomska, Julia Breuer, Hanna Ehnis, Renato Frischknecht, Anna Fejtová, Hanna Regus-Leidig, Johann Helmut Brandstätter, Kaspar Gierke · FENS Forum 2024
Synaptic ribbons (SRs) are the presynaptic hallmark of sensory ribbon synapses. SRs tether up to several hundred synaptic vesicles (SVs) to support continuous exocytosis. How tethering of SVs to SRs is achieved is currently unknown. We previously demonstrated that Piccolino, an integral component of SRs1,2, binds to RIBEYE, the main component of SRs, via its C-terminus3. Here, we explore how the N-terminus of Piccolino might interact with SVs. First, we ultrastructurally analyzed the distribution of SVs at photoreceptor ribbon synapses in Piccolino knock-out (KO) mice. We found a decrease in t
ePoster · Neuroscience