Characterisation of Magi-family synaptic scaffolding proteins in human iPSC-derived neurons
Maximilian Borgmeyer, Julia Knocks, Tomas Fanutza, Lukas Einhäupl, Doris Lau, Christian Wozny, Nina Wittemayer · FENS Forum 2024
Synapse formation is critical for the wiring of neural circuits in the developing brain. The composition and function of synapses has predominantly been studied in rodent cells. Here we show a novel protocol to generate human iPSC-derived forebrain-like neurons that show rapid synaptic development. To achieve this, we use an astrocyte feeder layer attached to a complex mix of ECM-components. Neuronal stem cells (NCS) are plated on this layer for maturation into neurons. We use this protocol to provide an initial characterisation of Magi-family synaptic scaffolding proteins in human neurons. Bo
ePoster · Neuroscience