ePoster · Neuroscience
Transcriptional co-development in human iPSC-derived astrocytes and neurons
Maurits Unkel, Bas Lendemeijer, Hilde Smeenk, Erik Bindels, Witte Hoogendijk, Femke de Vrij, Steven Kushner · FENS Forum 2024
Astrocytes and neurons collaboratively shape neural networks, influencing metabolism, synapse formation, and network dynamics. Dysfunctions in either cell type are implicated in diverse brain disorders. In order to gain insight into the transcriptional profile of hiPSC-derived astrocytes and neurons, we performed scRNA analysis and compared astrocytes and neurons before and after co-culture.Sequencing with Illumina Novaseq6000, preprocessing with 10x Genomics CellRanger. Custom R/Seurat pipeline for data analysis.NGN2 neurons were identified as excitatory. Within the astrocyte population we ob