Topic: serotonin dynamics

For decades, the molecule Serotonin is of interest not only but also because of its various functions within the brain. While the direct measurement of serotonin release in the brain is challenging, new genetically encoded sensors for serotonin opened new possibilities to measure release of serotonin in a high temporal and spatial resolution. Here we report the newest developments of one of these genetically encoded sensors for serotonin, called sDarken.Based on the initial published sDarken (Kubitschke et al. 2023) we now utilized mutagenesis, to develop a new color shifted variant which show