Multi-region calcium imaging in freely behaving mice with ultra-compact head-mounted fluorescence microscopes
Feng Xue, Fei Li, Ke-ming Zhang, Lufeng Ding, Yang Wang, Xingtao Zhao, Fang Xu, Danke Zhang, Mingzhai Sun, Pak-Ming Lau, Qingyuan Zhu, Pengcheng Zhou, Quo-Qiang Bi · FENS Forum 2024
To investigate the circuit-level neural mechanisms of behavior, simultaneous imaging of neuronal activity in multiple cortical and subcortical regions is highly desired. Miniature head-mounted microscopes offer the capability of calcium imaging in freely behaving animals. However, implanting multiple microscopes on a mouse brain remains challenging due to space constraints and the cumbersome weight of the equipment. Here, we present TINIscope, a Tightly Integrated Neuronal Imaging microscope optimized for electronic and opto-mechanical design. With its compact and lightweight design of 0.43 g,
ePoster · Neuroscience