Topic: Single Cell RNAseq

In the adult brain, subsets of astrocytes act as neural stem cells in two specialized niches, the sub-ventricular zone and hippocampal dentate gyrus, where they produce olfactory bulb interneurons and dentate gyrus granule cells throughout life. In specific conditions the genesis of new neurons can occur also outside these niches in the adult brain parenchyma. This ectopic neurogenesis is prominent in the striatum where it occurs in both physiologic, in adult rabbits and adolescent guinea pigs, or pathologic conditions, such as mouse models of both progressive and acute neuronal degeneration.

Ageing is the greatest risk factor for cognitive decline and neurodegeneration. A characteristic of ageing is the loss of proteostasis. Neddylation maintains proteome homeostasis, it is involved in neuronal differentiation and its deregulation is linked to neurodegenerative diseases. The role and mechanisms of Neddylation with physiological ageing remain still unclear.We studied the expression and impact of neddylation on ageing and cognitive decline. We found that neddylation increases with age in the hippocampus neurogenic niche both in human and mouse samples. Aged, but not young, Nae1+/- h

The dentate gyrus (DG) of the ventral hippocampus is a key hub critical for the responses to prolonged chronic stress and antidepressant drugs, including the antidepressant candidate acetyl-L-carnitine (LAC). Prior studies from our and other laboratories showed transcriptome-wide signatures in this brain area with robust changes in genes important for neuronal plasticity, including the metabotropic glutamate receptor-2 (mGlu2), and insulin signaling pathways after chronic stress, and that these changes are ameliorated by short-term administration of LAC. Here, we used 10X Genomics droplet-base