Chronic exposure to glucocorticoids during critical neurodevelopmental periods leads to lasting shifts in neuronal type distribution and overall brain architecture
Ilknur Safak Demirel, Pia Giraudet, Malgorzata Grochowicz, Anthi C. Krontira, Leander Dony, Tim Schäfer, Elisabeth Binder, Cristiana Cruceanu · FENS Forum 2024
The brain undergoes important growth and plasticity during prenatal development. Altered activation of the glucocorticoid receptor (GR) system is one of the factors mediating stress effects during this time, likely through transcriptional dysregulation. To investigate these processes in a human-specific in vitro system, we used induced pluripotent stem cell-derived 3-dimensional brain organoids. To determine cell-type specific GR activation response, we profiled the transcriptomes of thousands of individual cells using single-cell transcriptomic analyses following glucocorticoids exposure acut
ePoster · Neuroscience