Topic: single-nuclei RNA-seq

Dravet Syndrome is a developmental and epileptic encephalopathy, with intractable seizures and other comorbidities; due to loss of function variants in SCN1A, the disease mostly manifests because of the impairment of the functionality of inhibitory interneurons. Trying to address the unmet clinical needs of patients carrying missense variants, we used a transgenic mouse model carrying the recurrent variant p.(Ala1783Val) in Scn1a. This mouse closely recapitulates the clinical phenotype, allowing to more deeply explore the epileptogenesis and find new precision medicine approaches.We carried ou

Memory is the capacity of an organism to acquire, store and recover information based on experience. In the brain, experiences that become memories appear as enduring changes only in a small set of activated neurons, called engrams.While the mechanisms of how such memories are deposited in engrams for short periods of time has received considerable attention over the past decade, the current knowledge of how memories are stored in engram cells in the long run is still scarce. Consequently, this project aims to identify the mechanisms based on which a memory becomes remote by integrating a wide