Topic: skeletonization

Dendritic spines are key structures for neural communication, learning and memory. Spine size and shape probably reflect learning and synaptic strength. With superresolution STED microscopy (STimulated Emission Depletion microscopy) we imaged for the first time in 3D the detailed shape of the majority of the spines of individual neurons in turtle cortex (Trachemys scripta elegans) (Figure, scale bars: upper row 0.5 µm, lower row 1 µm). A combination of skeletonization in 3D and segmentation of almost 7000 spines allowed us to describe their morphology in detail. To classify the spines, we clus