Cocaine detrimentally affects mitochondrial functionality and cell viability in dopaminergic neurons
Pablo Zamorano Gonzalez, Luca Bandini, Nadia Valverde, Silvia Claros, Yanina Romero-Zerbo, Estrella Lara, Luis Santín, Elisa Martín-Montañez, Belén Gago*, María García Fernández* · FENS Forum 2024
An elevated consumption of cocaine (benzoylmethylecgonine), which causes anesthetic and stimulant effects on the central nervous system, may be associated with several neurodegenerative conditions affecting dopaminergic neurons, such as Parkinson's disease (PD). To investigate the impact of cocaine on cell viability and morphology, dopaminergic neurons from the substantia nigra (SN4741) were cultured. Analysis involved assessing cell death (LDH levels) and cell morphology (GIEMSA staining) after a 24-hour treatment period. Additionally, the effects on reactive oxygen species (ROS) generation (
ePoster · Neuroscience