Spatial proteomics reveals distinct protein patterns in cortical migration disorders caused by LIN28A overexpression and WNT activation
Jelena Navolic, Sara Hawass, Maximilian Middelkamp, Manuela Moritz, Jan Hahn, Antonia Gucke, Piotr Sumislawski, Lisa Ruck, Christoph Krisp, Matthias Dottermusch, Shweta Godbole, Hartmut Schlüter, Julia E. Neumann · FENS Forum 2024
LIN28A is an RNA-binding protein and stem cell marker, and its overexpression was described in rare malignant brain tumours of early childhood. CTNNB1 is an effector of the WNT pathway which plays important roles during development and activating mutations have been reported in malignant brain tumours. In order to investigate the interplay of these oncogenic proteins during embryonal brain development, we overexpressed both in murine neural progenitor cells in vivo.The sole overexpression of either LIN28A, stabilized Ctnnb1 or the combination of both in hGFAP-positive forebrain precursor cells
ePoster · Neuroscience