Topic: superficial dorsal horn

Intractable chronic itch is resistant to antihistamines, and difficult to treat, which significantly reduces quality of life. Here, we focused on GABA transporter 1 (GAT1) whose blockade might have an antipruritic effect by increasing endogenous GABA.The allergic contact dermatitis model of chronic itch was generated by applying 1-fluoro-2,4-dinitrobenzene (DNFB) onto the shaved neck skin using ICR male mice. Five days after sensitization by topical application of 50 µL 0.5% DNFB solution, mice were challenged repeatedly with 30 µL 0.25% DNFB solution on day 1, 3, 5, and 7. Scratching frequenc