Wiring diagram of a central sensory projection revealed by dense Brainbow labeling
Katie Matho,Minh Son Phan,Lamiae Abdeladim,Nelly Vuillemin,Dragos Niculescu,David Mou,Morgane Roche,Marie Guirguis,May Zhang,Laura Dumas,Francesco Boato,Pierre Mahou,Ignacio Arganda-Carerras,Jonathan Bradley,Willy Supatto,Alexis-Pierre Bemelmans,Jeff Lichtman,Anatole Chessel,Emmanuel Beaurepaire,Jean Livet · COSYNE 2022
Sensory features are encoded in topographically mapped neuronal populations in the brain. Their fine structure layout is not readily accessible due to their dense wiring and extended volume. To reveal their wiring logic, we combine genetic multicolor labeling of neural cells and large-volume micrometer scale imaging with color multiphoton microscopy. We present an analysis at single neuron resolution of the medial nucleus of the trapezoid body (MNTB), an essential relay for sound localization, which receives inputs from the cochlear nucleus (CN). Multicolor multiphoton microscopy allowed us to
Fri, Mar 18, 2022