Topic: synaptotagmin 1

Establishing synaptic connections between neurons is essential for neuronal circuitry and efficient brain communication While the role of neuronal activity in refining and maintaining synapses is well known, we focus on its role, specifically spontaneous neurotransmitter release, for synapse formation between dentate gyrus axons, the mossy fibres (MF), onto CA3 pyramidal cells in the hippocampus. Neurotransmitter release occurs through two primary modes: action potential induced release and spontaneous fusion of synaptic vesicles. These modes are underpinned by distinct pools of vesicles, exhi

Synaptic transmission is a complex process, which requires activation of presynaptic Ca2+ channels, Ca2+ influx and binding to release sensors. Synaptotagmin 1 (Syt-1) has been previously identified as the primary Ca2+ sensor for fast synchronous transmitter release, while synaptotagmin 7 (Syt-7) was suggested to mediate slower, asynchronous release. However, recent reports have conflicting conclusions on the mechanisms of vesicle release modes, making our understanding of Ca2+ sensing in transmission unclear. To understand the action of synaptotagmins at a central synapse, we performed subcel