Loss of the presynaptic scaffold Piccolo reduces Ca2+ sensitivity of glutamate release and short-term plasticity in small brain synapses
Anke Boerner, Debarpan Guhathakurta, Kaspar Gierke, Bartomeu Perelló-Amorós, Enes Yağız Akdaş, Renato Frischknecht, Johann Helmut Brandstätter, Anna Fejtova · FENS Forum 2024
Calcium-evoked exocytosis of synaptic vesicles at the presynapse involves recruitment, docking, and priming of synaptic vesicles as well as localization of voltage-gated calcium channels in their close proximity. Piccolo, a large multidomain protein, acts as a scaffolding protein at the active zone, thus mediating the exact localization and functional interaction of several involved proteins. We aimed to investigate the functional role of piccolo, namely the effect on glutamate release and the involvement of calcium channel regulation, using cortico-hippocampal cell cultures from a novel condi
ePoster · Neuroscience