Brain microvessel-specific deletion of the COL4A1 gene in adult mice leads to widespread microhemorrhages resembling human microbleeds
Hyunmi Kim, Jae Young Lee, Byung Gon Kim · FENS Forum 2024
Aims: Type 4 collagen encoded by COL4A1 gene is an integral component of blood-brain barrier (BBB). It has been reported that mutations in COL4A1 can cause various abnormalities in human cerebral microvasculature. We sought to determine the pathological consequences of COL4A1 deficiency in brain microvessels of adult mice. Methods: To knock out COL4A1 gene specifically in brain endothelial cells (BECs) of adult mice, we combined an adeno-associated virus to target the brain endothelium (AAV-BR1) with CRISPR/Cas9 system. The gRNA targeting exon 1 of COL4A1 was selected, packaged with AAV-BR1, a
ePoster · Neuroscience