HIV-1 Tat protein induces inhibition of serotonin transporter in the midbrain and increases serotonin release dynamics in the substantia nigra of inducible Tat transgenic mice
Jun Zhu, Ana C Jimenez Torres, Sarah E Davis, Jay McLaughlin · FENS Forum 2024
Dysregulation of serotonin (5-HT) transmission has been implicated as a factor in the development of depression. This study determined the effects of Tat on serotonin transporter (SERT), dynamic 5-HT release, and the effect of induction of Tat expression on mobility in the tail-suspension test (TST) in inducible Tat transgenic (iTat-tg) mice. Optimization of recombinant Tat1-86 (rTat1-86) concentration revealed a Ki of 7.2 nM for it inhibiting 5-HT uptake into mouse synaptosomes. SRI-32743 displayed a partial antagonism with 13 nM affinity and 69.5% Emax for inhibiting 5-HT uptake in CHO cells
ePoster · Neuroscience