Adeno-associated viral tools to trace neural development and connectivity across amphibians
Eliza C.B. Jaeger, David Vijatovic, Astrid Deryckere, Nikol Zorin, Akemi L. Nguyen, Georgiy Ivanian, Jamie Woych, Rebecca C. Arnold, Alonso Ortega Gurrola, Arik Shvartsman, Francesca Barbieri, Florina Alexandra Toma, Gary J. Gorbsky, Marko E. Horb, Hollis T. Cline, Timothy F. Shay, Darcy B. Kelley, Ayako Yamaguchi, Mark Shein-Idelson, Maria Antonietta Tosches, Lora B. Sweeney · FENS Forum 2024
Amphibians hold a unique position in the study of the evolution and development of the vertebrate the central nervous system (CNS), as they reflect the transition from aquatic to land-based locomotion. A comprehensive exploration of the amphibian CNS, however, necessitates genetic and neural tracing tools, which were previously limited.Adeno-associated viruses (AAVs) have gained popularity in mammals, due to lack of toxicity and host genome integration. Aiming to add AAVs to the amphibian toolkit, we tested AAV serotypes for CNS transduction in three distantly-related amphibian species: the fr
ePoster · Neuroscience