Long poly(A) tails of neuropeptide mRNAs originate in the cytoplasm of neurosecretory cells
Bartosz Tarkowski, Hanna Grzesik, Wiktoria Orzeł, Paweł Krawczyk, Monika Pawłowska, Olga Gewartowska, Karolina Piechna, Sebastian Jeleń, Jan Brancewicz, Kamil Jachacy, Jakub Gruchota, Marcin Szpila, Katarzyna Sałas, Sara Domagała, Andrzej Dziembowski · FENS Forum 2024
Specialized secretory neurons in the hypothalamus and the Edinger-Westphal nucleus secrete severalneurohormones. Very little is known about the regulation of their expression at the post-transcriptionallevel. Here we discovered that a non-canonical cytoplasmic poly(A) polymerase Tent5a is a globalpositive regulator of neurohormone expression. We detect Tent5a in Edinger-Westphal, paraventricularand supraoptic nuclei, as well as the lateral hypothalamic area. Importantly, RNA sequencing of theseregions’ biopsies, as well as northern analysis of the whole hypothalami, revealed decreased expressi
ePoster · Neuroscience