SH-SY5Y cell differentiation to dopaminergic neuron-like cells using retinoic acid
Simas Janutenas, Vassileios Stratoulias, Umamaheswari Umasankar, Silvia Orsini · FENS Forum 2024
Background: We study dopaminergic neurons with specific emphasis on Parkinson’s disease. Our work involves implementing procedures for examining in vitro mouse primary dopaminergic neurons as well as dopaminergic neurons derived from human stem cells. Here we want to establish a continuous cell line model for fast and cost-effective production of human dopaminergic-like cells to be used in our studies. Aims: Differentiate SH-SY5Y cells into dopaminergic-like cells using Retinoic Acid. Optimize the differentiation protocol for highest yield of TH positive cells. Methods: Retinoic Acid is used t
ePoster · Neuroscience