Molecular connectomics reveals a glucagon-like peptide 1 sensitive neural circuit for satiety
Addison Webster, Jordan Becker, Chia Li, Dana Schwalbe, Damien Kerspern, Eva Karolczak, Elizabeth Godschall, Dylan Belmont-Rausch, Tune H. Pers, Andrew Lutas, Naomi Habib, Ali D. Guler, Michael J. Krashes, John N. Campbell · FENS Forum 2024
Liraglutide and other agonists of the glucagon-like peptide 1 receptor (GLP-1RAs) are effective weight loss drugs, but how they suppress appetite remains unclear. GLP-1RAs inhibit hunger-promoting Agouti-related peptide (AgRP) neurons of the arcuate hypothalamus (Arc) but only indirectly, implicating synaptic afferents to AgRP neurons. To investigate, we developed a new method combining monosynaptic rabies with single-cell transcriptomics to identify connected neurons in a genetically tractable manner: “RAMPANT” (Rabies Afferent Mapping by Poly-A Nuclear Transcriptomics). Applying RAMPANT to A
ePoster · Neuroscience