Topic: TTC staining

First, on a cell model, primary cortical neurons were used to determine the safe dose of ginsenoside by performing toxicity assays with different concentration gradients of ginsenoside using MTT assay; Then pharmacodynamic screening was performed using primary cortical neuron oxygen-glucose deprivation reperfusion (OGD/R), and primary cortical neurons cultured to day 7 were replaced with sugar-free DMEM after drug preadministration for 2h.Then the neurons were placed in a hypoxic chamber containing 95% N2 and 5% CO2 for 4 h, and after that they were switched to the normal medium containing gin