Topic: two-photon Ca2+ imaging

Sensory information is first processed by various thalamic nuclei before reaching the pallium in amniotes, where it is used for higher-order computations such as integrating diverse sensory information that can be utilized to form associations. In teleost fish, the dorsal thalamic nuclei, believed to be analogous to the amniote thalamus, projects primarily to the subpallium. However, the major diencephalic input to the pallium originates from the preglomerular nucleus (PG), suggesting that it may perform analogous functions to the amniote thalamocortical pathway. To examine this, we first mapp

Hippocampal pyramidal cells (PCs) express spatially tuned activity (i.e., ‘place cells’ exhibiting ‘place fields’, PF) to support navigation. However, the cellular mechanisms of novel PF formation are elusive. Behavioral time scale synaptic plasticity (BTSP), recently described in mouse CA1PCs, is a putative mechanism of PF formation (Bittner et al., 2015). It starts with a silent PC exhibiting a large Ca2+ plateau in dendrites accompanied by somatic bursting, which initiates the emergence of tuning near the location of induction. Consequently, activity is strongest during formation and lower

The hippocampus supports flexible navigation by creating context-dependent spatial maps. How these maps emerge during learning and change upon manipulation of the context is not well understood. To elucidate these dynamics, we studied CA1 pyramidal cell (PC) representations in a contextual go/no-go task.We trained water-restricted, head-fixed Thy1-GCaMP6s mice to run on a treadmill in virtual environments, and distinguish two visually distinct corridors by selectively licking in a reward zone in one corridor. We monitored CA1PC activity by two-photon Ca2+ imaging and assessed behavior by measu

In this study, we investigate how contextual memory is shaped by observational fear learning during encoding and recall. We are imaging neurons in layer 2/3 of the retrosplenial cortex in GCaMP6s mice with two-photon Ca2+ imaging using a head-fixed observational fear learning paradigm in virtual reality. The animals are surrounded by three screens and run on a running wheel as they travel through 3 different VR environments. In two of the environments, we play recordings of mouse vocalisations emitted while they were receiving foot shocks, and a neutral sound in the third environment. A single

Animals continuously evaluate past and current sensory and contextual information to make appropriate decisions during behaviour. How animals employ specific behavioural strategies while learning a task and how the orbitofrontal cortex (OFC) contributes to such strategy-based learning remains understudied. To investigate this, we trained mice on a tactile reversal learning task and implemented a trial-resolution Bayesian evidence accumulation model to measure the probability of the strategies used. We analysed multiple exploratory strategies across key task-learning phases and subsequently aft

Sensory information is first processed by various thalamic nuclei before reaching the pallium in amniotes where it is used for higher-order computations such as encoding new memories. In teleost fish, the dorsal thalamic nuclei, believed to be analogous to the amniote thalamus, projects primarily to the subpallium. However, the major diencephalic input to the pallium originates from the preglomerular nucleus (PG), suggesting that it may perform analogous functions to the amniote thalamocortical pathway. To examine this, we first mapped the anatomical inputs to the zebrafish PG and showed that