Topic: two-photon fluorescence microscopy

To study the neural correlates of naturalistic behaviors, we seek to record neural activity in unrestrained animals. Due to behavior-induced motion artifacts, such recordings are particularly challenging for two-photon fluorescence microscopy. Studying multi-neuronal activity in the central nervous system in moving larval Drosophila, whose brain is translated, rotated, and deformed in 3D, has been a great challenge. To overcome the challenges, we developed a method capable of calcium recording from multiple neurons in a brain of a freely moving Drosophila larva with extremely low latency (100