Optical recording of unitary synaptic connections between CA3 pyramidal cells using Voltron imaging
Róbert Layous, Árpád Mike, Bálint Tamás, Eszter Sipos, Antónia Arszovszki, János Brunner, Janos Szabadics · FENS Forum 2024
Understanding synaptic connectivity is crucial for understanding how neuronal networks contribute to specific brain functions. However, current methods can measure the functionality only of a limited number of synaptic connections in sufficient detail. Therefore, we evaluated the applicability of a voltage imaging method in acute rat hippocampal slices to map unitary synaptic connections between CA3 neurons by using Voltron, which can detect both action potentials and small subthreshold events, such as unitary EPSPs. These connections are crucial for major hippocampal memory functions; however
ePoster · Neuroscience