RatDISCO, an optical clearing and immunolabelling pipeline for large rat (and mice) tissue
Cristina Martinez Gonzalez, Kirsty Craigie, Sally Till, Britt Van De Gevel, Peter Kind, Nathalie Rochefort, Ian Duguid, Matt Nolan · FENS Forum 2024
Understanding brain function in health and disease requires unbiased three-dimensional mapping of its neurochemical architecture. Traditional histological methods require mechanical tissue sectioning, leading to cutting artefacts, lengthy processing times and loss of crucial three-dimensional information. To overcome that, optical clearing techniques have soared since 3DISCO allowed us to visualise neuronal networks in the whole mouse brain1. Immunolabelling in whole organs is possible; however, antibody penetration is a challenge and most clearing protocols are tailored for mice or human tiss
ePoster · Neuroscience