Phospholipase gamma2: A genetic risk factor for Alzheimer's disease that alters the synaptic input in granular cells through alterations in mossy fiber boutons
Avishek Roy, Anaël Erhardt, Severine Deforges, Christophe Mulle · FENS Forum 2024
PhospholipaseC γ2 (PLCG2) is known to have direct link with genetic risk factors for Alzheimer’s like dementia (AD). Previous work showed its action in AD is regulated through microglia. However, the synaptic/ electrophysiological correlates are elusive. In present study, WT mouse were transfected with sh-RNA of LV-PLCG2 (U6 promotor, GFP as reporter; shRNA) or scrambled (used as control; shNT). Whole-cell voltage-clamp recordings were performed on acute slices with glass pipette (<3-4MΩ) at -70mv, followed by morphometry. Two-tailed Mann-Whitney test was used for statistics unless otherwis
ePoster · Neuroscience