Analysis of the impact of MnCl2 present in atmospheric particulates on synaptic development using brain models based on hiPSCs derived neurons
Erica Debbi, Chiara D'Antoni, Federica Cordella, Silvia Ghirga, Silvia Di Angelantonio, Nicolas Baeyens · FENS Forum 2024
Air pollution is a significant threat to human health and it is estimated that globally 8.9 million deaths happen because of it. A link between urban particulate matter exposure and an increased risk of neurodegenerative disease has already been identified. For this reason, I used wild-type hiPSCs to set up a 2D differentiation protocol of about 70 days. Cultures were untreated or treated with two doses of manganese, 1 μM and 20 μM, chosen in relation to scientific literature and after a MTS viability assay. Treatments started from day 40 until the end of differentiation (intermediate time poi
ePoster · Neuroscience