Topic: XAV939

We are investigating the developmental origins of alterations in striatal interneurons observed in brain of patients diagnosed with Tourette’s Syndrome using organoid models cultured from patient-derived induced pluripotent stem cells. Here we provide a first characterization of organoids differentiated using a new protocol aimed to induce ventral forebrain fates in 7 iPSC lines from neurotypical adults. During neural induction with dual SMAD inhibition, patterning was carried out over 5 days using the Wnt-antagonist XAV939 alone in our standard dorsal forebrain protocol, and in combination wi