Poster preview
ePoster
ESTABLISHING A CELLULAR AND IN-VIVO MODEL FOR MIMICKING LEWY BODY PATHOLOGY
Shujin Heand 15 co-authors
Charité – Universitätsmedizin
FENS Forum 2026 (2026)
Barcelona, Spain
Board PS04-08PM-250
Presentation
Date TBA
Board: PS04-08PM-250
Event Information
Poster Board
PS04-08PM-250
Poster
View posterAbstract
Lewy bodies (LBs) are the histopathological hallmark of Parkinson's disease (PD), with α-synuclein being the major proteinaceous component. The relationship between LB formation and the death of dopaminergic neurons is complex and incompletely understood. It has recently been shown that α-synuclein abnormally aggregates and interacts with other components such as proteins and membranous organelle within neurons. In order to further characterize the formation and pathological role of LBs, we employed the concept of liquid-liquid phase separation to generate a minimal-system where α-synuclein forms aberrant phase separation, which we termed LB-like structures (LBLs). Specifically, by ectopically co-expressing α-synuclein and synphilin-1 in non-neuronal cells, we observed LBLs that exhibited similar physical and biochemical properties to LBs. Specifically, using fluorescence-based microscopy and soft X-ray tomography, we demonstrate the accumulation of membranous organelles, including mitochondria, at the interface of LBLs. In our latest efforts, we were able to recapitulate these findings in-vivo under stressed conditions, using Caenorhabditis elegans as a model. Furthermore, we observed animal mobility dysfunction in the affected worms. Together, our results highlight the value of LBLs as cellular and in-vivo model, potentially enabling the discovery of novel therapeutic strategies, targeting the molecular pathophysiology of PD.
Recommended posters
DISSECTING THE INTERACTION BETWEEN THE ABERRANT PHASE SEPARATION OF ALPHA-SYNUCLEIN AND ORGANELLE MISLOCALIZATION
Ivona Sukunda, Aleksandr Korobeinikov, Christian Hoffman, Jian-Hua Chen, Asima Nayak, Daniele Bano, Pallavi Gopal, Carolyn Larabell Larabell, Dragomir Milovanovic
TARGETED DEGRADATION OF ΑLPHA-SYNUCLEIN LIMITS PATHOLOGICAL AGGREGATION IN A CELLULAR MODEL OF PARKINSON’S DISEASE
Rim Ayach, Anna Chami, Claire Tronel, Sylvie Chalon, Elisa Chenaf, Frédéric Buron, Sylvain Routier, Hervé Boutin
EARLY Α-SYNUCLEIN PATHOLOGY IN MICE LEADS TO MOTOR DYSFUNCTION, DIFFERENTIAL MICROGLIA CLUSTERS AND ALTERED IMMUNE SIGNALING
Frederik Rasmussen, Sofie Frandsen, Elisa Pfannenmueller, Maria Vasconcelos, Yogita Sharma, Sofie Fonager, Søren Degn, Johan Jakobsson, Anna Klawonn
TRIPLE ASSEMBLOIDS TO MODEL CORTICO-STRIATAL AND NIGRO-STRIATAL CONNECTIVITY IN PARKINSON’S DISEASE
Pauline Lachal, Christiane Zhu, Gabin Devely, Evodie Lassalle, Anaelle Pinçon, Benjamin Galet, Olga Corti, Hélène Cheval, Philippe Ravassard
UNRAVELING THE CONSEQUENCES OF INDUCED SYNUCLEINOPATHY IN DOPAMINERGIC AND NORADRENERGIC NEURONS
Hanna Vila Merkle, Alexia Lantheaume, Maria Gruber, Ilvia Rodriguez-Rozada, Michael Schellenberger, Konstantin Kobel, Kilian Katzenberger, Philip Tovote
STUDY OF THE PATHOLOGICAL EFFECT OF THE NOVEL RS199645736G>A MUTATION IN THE HUMAN SYN3 GENE
Francesco Amodeo, Francesca Longhena, Gaia Faustini, Alessandro Padovani, Giorgio Biasiotto, Andrea Pilotto, Arianna Bellucci