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HYDROGEL-DELIVERED STROMAL VASCULAR FRACTION FOR SPINAL CORD REPAIR
Enrico Frigerioand 6 co-authors
IRCCS - "Mario Negri" Institute for Pharmacological Research
FENS Forum 2026 (2026)
Barcelona, Spain
Board PS04-08PM-097
Presentation
Date TBA
Board: PS04-08PM-097
Event Information
Poster Board
PS04-08PM-097
Poster
View posterAbstract
Aim: Spinal cord injury (SCI) remains difficult to treat due to poor regenerative capacity. While adipose-derived Stromal Vascular Fraction (SVF) offers therapeutic potential through inflammation modulation and tissue repair, its clinical use is hindered by low cell retention at the injury site. This study aimed to develop a biocompatible hydrogel to encapsulate SVF, enhancing cell viability and engraftment.
Methods: We engineered a lyophilized agarose–carbomer and polyethylene glycol hydrogel. In vitro experiments assessed the platform's ability to support SVF populations over 10 days. Cell viability was measured using CFDA-SE and propidium iodide staining, while live immunocytochemistry and colocalization microscopy identified specific subpopulations, including ADSCs, endothelial cells, and pericytes.
Results: The SVF-laden hydrogels maintained high cell viability throughout the 10-day culture period. Results confirmed the sustained presence and survival of key regenerative populations, particularly ADSCs, within the hydrogel matrix, suggesting a highly favourable microenvironment for cell retention.
Conclusion: Encapsulating SVF in this specialized hydrogel promotes prolonged in vitro viability. This delivery strategy represents a promising advancement for enhancing the efficacy of regenerative therapies in SCI.
Methods: We engineered a lyophilized agarose–carbomer and polyethylene glycol hydrogel. In vitro experiments assessed the platform's ability to support SVF populations over 10 days. Cell viability was measured using CFDA-SE and propidium iodide staining, while live immunocytochemistry and colocalization microscopy identified specific subpopulations, including ADSCs, endothelial cells, and pericytes.
Results: The SVF-laden hydrogels maintained high cell viability throughout the 10-day culture period. Results confirmed the sustained presence and survival of key regenerative populations, particularly ADSCs, within the hydrogel matrix, suggesting a highly favourable microenvironment for cell retention.
Conclusion: Encapsulating SVF in this specialized hydrogel promotes prolonged in vitro viability. This delivery strategy represents a promising advancement for enhancing the efficacy of regenerative therapies in SCI.
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