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A NOVEL MINIATURIZED TWO-PHOTON MICROSCOPE FOR FLUORESCENCE LIFETIME IMAGING REVEALS SUSTAINED CAMKII AND CREB INTRACELLULAR SIGNALING IN FREELY BEHAVING ANIMALS
Taddeo Salemiand 3 co-authors
Max Planck Florida Institute for Neuroscience
FENS Forum 2026 (2026)
Barcelona, Spain
Board PS07-10AM-004
Presentation
Date TBA
Board: PS07-10AM-004
Event Information
Poster Board
PS07-10AM-004
Poster
View posterAbstract
Neuronal signaling operates across multiple timescales to encode, store, and integrate experience. However, the dynamics of these molecular processes in freely behaving animals remain largely unknown. Here, we introduce a miniature two-photon fluorescence lifetime imaging microscope (mini-2pFLIM) that enables subcellular imaging of FRET biosensors in unrestrained mice. Using sensors for CaMKII and CREB, we monitored kinase activity in dendritic and nuclear compartments during environmental enrichment. Both CaMKII and CREB showed sustained activation lasting more than 30 minutes and exhibited significant basal activity even at rest. These findings reveal that intracellular signaling is not a series of transient bursts but maintains a continuous tone that spans seconds to hours. Our results uncover a molecular bridge between fast synaptic and slow nuclear processes and demonstrate how mini-2pFLIM enables direct visualization of multi-timescale biochemical signaling underlying experience-dependent plasticity.
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