Poster preview
ePoster
SPATIALLY PRECISE IN SITU TRANSCRIPTOMICS IN NPF NEURONS
Yaara Diamant Karasikand 3 co-authors
The Alexander Kofkin Faculty of Engineering, Bar-Ilan University
FENS Forum 2026 (2026)
Barcelona, Spain
Board PS01-07AM-176
Presentation
Date TBA
Board: PS01-07AM-176
Event Information
Poster Board
PS01-07AM-176
Poster
View posterAbstract
Spatial organization of RNA within neurons plays a critical role in regulating neuronal function and behavior, yet existing transcriptomic approaches are largely limited to either RNA sequencing without spatial information, or spatial examination of thin tissue sections. These approaches disrupt three-dimensional tissue architecture, which is especially limiting when studying neuronal populations distributed across the intact brain. Here, we apply Expansion Sequencing (ExSeq), which combines expansion microscopy and in situ sequencing, to the entire Drosophila brain. Using this approach, we mapped the spatial localization of 100 genes within neuropeptide F (NPF) neurons, a distributed neuronal population in the Drosophila brain. Because ExSeq is applied to the intact brain, transcript localization can be resolved at subcellular scales, including the soma, dendrites, axons, and synaptic regions of NPF neurons.
Recommended posters
SUPER-RESOLVED SPATIAL TRANSCRIPTOMICS REVEALS EARLY HIPPOCAMPAL RNA LOCALIZATION CHANGES IN A MOUSE MODEL OF ALZHEIMER’S DISEASE
Hadar Eger, Yaara Karasik
HIGH-THROUGHPUT SPATIAL TRANSCRIPTOMICS ON 50 UM-THICK VIBRATOME SECTIONS TO ENABLE INTEGRATION WITH TWO-PHOTON CALCIUM IMAGING
Morgane Storey, Alireza Gargoorimotlagh, Luca Fusar Bassini, Paul Shuker, Maxwell Shinn, Isabelle Prankerd, Zhiyao Zhou, Kenneth Harris, Gioele La Manno, Carl C.H. Petersen
SPATIAL TRANSCRIPTOMIC PROFILING REVEALS MOLECULAR SIGNATURES OF PERINEURONAL NET-ASSOCIATED PARVALBUMIN INTERNEURONS
Sverre Grødem, Guro Vatne, Marianne Fyhn
ESTABLISHING A NOVEL PROTOCOL FOR THE TRANSCRIPTOMIC PROFILING OF NEURONS ENCAPSULATED BY A PERINEURONAL NET IN THE HUMAN BRAIN
Júlia Alcaide, Dominique Lumley, Claudia Belliveau, Chen Gang, Gabriella Frosi, Maria Antonietta Davoli, Gustavo Turecki, Naguib Mechawar
LIGHT-INDUCIBLE PROXIMITY LABELLING IN VIVO CAPTURES SEX-SPECIFIC RNA AT EXCITATORY SYNAPSES
Joshua Davies, Lin John Yu-luen, Bredy Timothy, Marshall Paul
EXPANSION MICROSCOPY ENABLES SYNAPTIC-RESOLUTION IMAGING OF THE ZEBRA FINCH SONG SYSTEM ACROSS DEVELOPMENT
Andreea-Maria Aldea