Poster preview
ePoster
SPATIO-TEMPORAL ANALYSIS OF LOW- AND HIGH-FREQUENCY ACTIVITY IN EX-VIVO HUMAN TISSUE USING HIGH-DENSITY MICRO-ELECTRODE ARRAYS
Giulia Maria Boianiand 11 co-authors
University of Modena and Reggio Emilia
FENS Forum 2026 (2026)
Barcelona, Spain
Board PS05-09AM-384
Presentation
Date TBA
Board: PS05-09AM-384
Event Information
Poster Board
PS05-09AM-384
Poster
View posterAbstract
Extracellular recordings by High-Density Micro-Electrode Arrays (HD-MEAs) enable the investigation of human cortical and hippocampal circuits activity at both single-cell and network levels (Emery et al., 2024). We mechanically coupled HD-MEAs with human brain tissue slices, obtained from surgical resections of patients with focal epilepsy. Neuronal activity was measured under artificial cerebrospinal fluid (aCSF) as well as modified aCSF to elicit epileptic-like activity and thus enabling micro-scale mapping of network activity.
Extracellular raw voltage recordings were filtered to identify multiunit activity (MUA) and Local Field Potentials (LFP). Spatiotemporal propagation patterns and spectral features of both spiking and LFP activity were then jointly analyzed to infer condition-dependent network organization and dynamic state transitions. Modified aCSFs promoted more frequent occurrence of detected events, spatially synchronized across HD-MEA microelectrodes, characterised by broader spatial correlations. Slow oscillations in LFPs were temporally aligned with the onset of MUA population bursts and revealed spatially dependent coupling to neuronal firing. Distinct LFP signal power distribution across frequency bands and throughout the tissue spatial extent further revealed a rich spatio-temporal organization and a tight interplay between spiking assemblies and network rhythms.
This work suggests that HD-MEA recordings play an important role for investigating human cortical and hippocampal network dynamics in vitro, complementing key clinical observations and offer access to cell- and microcircuit-level disease mechanisms and providing an opportunity for developing and testing new therapeutic strategies.
Recommended posters
FUNCTIONAL NETWORK PROPERTIES OF FRESHLY RESECTED HUMAN CORTICAL TISSUES FROM FOCAL EPILEPSY
Alessio Di Clemente, Irene Incerti, Giulia Maria Boiani, Manuela Tore, Laura Monni, Anna Maria Costa, Adam Armada-Moreira, Anna Elisabetta Vaudano, Elisa Moriconi, Giacomo Pavesi, Stefano Meletti, Daniela Gandolfi, Jonathan Mapelli, Michele Giugliano
A MULTIMODAL PIPELINE FOR EX-VIVO HUMAN BRAIN SLICES: INTEGRATING HD-MEA, 2-PHOTON IMAGING, AND PATCH-CLAMP ELECTROPHYSIOLOGY
Manuela Tore, Laura Monni, Anna Maria Costa, Alessio Di Clemente, Irene Incerti, Giulia Maria Boiani, Anna Elisabetta Vaudano, Elisa Moriconi, Giacomo Pavesi, Stefano Meletti, Daniela Gandolfi, Michele Giugliano, Jonathan Mapelli
MAPPING ELECTRICAL ACTIVITY OF NEURONAL NETWORKS AT SINGLE-CELL RESOLUTION USING A NOVEL UHD-CMOS MEA FIELD-POTENTIAL IMAGING
Naoki Matsuda, Han Xiaobo, Yokoi Remi, Chinatsu Suzuki, Bumpei Noda, Yamanaka Makoto, Ikuro Suzuki
ELECTROPHYSIOLOGICAL SIGNATURES IN ACUTE SLICES OF LIVING HUMAN EPILEPTOGENIC CORTEX AND HIPPOCAMPUS
Vera Lezhneva, Polina Abushik, Mireia Gomez-Budia, Anssi Pelkonen, Nataliia Novosolova, Liudmila Saveleva, Henri Eronen, Omar Narvaez, Mastaneh Torkamani-Azar, Tuomas Rauramaa, Arto Immonen, Ville Leinonen, Antti Huotarinen, Kuopio Epilepsy Center Epilepsy Surgery Group, Leena Jutila, Jussi Tohka, Olli Gröhn, Reetta Kälviäinen, Alejandra Sierra, Tarja Malm
FUNCTIONAL CHARACTERISATION OF HUMAN STEM CELL-DERIVED CORTICAL NETWORKS AND ORGANOIDS WITH CMOS HD-MEA AND CALCIUM IMAGING
Arkoprovo Paul, Ross Birks, Davide Ghio, Ho Fai Po, Laurissa Havins, Paul Roach, David Saad, Eric Hill, Rhein Parri
BURSTING NEURONAL ACTIVITY IN VIVO: INSIGHTS FROM PRE-SURGICAL MICROELECTRODE RECORDINGS IN PATIENTS WITH DRUG-RESISTANT EPILEPSY
Ágnes Kandrács, Brieg Oude, Anthony Pinto, Bertrand Mathon, Katia Lehongre, Valerio Frazzini, Vincent Navarro