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STANDARDIZATION OF A NEUROANATOMICAL PROTOCOL FOR THE DEVELOPMENT OF A BRAIN ATLAS IN NON-TRADITIONAL VERTEBRATES
Gabriela Gonzálezand 5 co-authors
University of Los Andes
FENS Forum 2026 (2026)
Barcelona, Spain
Board PS06-09PM-399
Presentation
Date TBA
Board: PS06-09PM-399
Event Information
Poster Board
PS06-09PM-399
Poster
View posterAbstract
Neuroanatomical atlases are fundamental tools for the study of brain organization; however, their availability is largely restricted to traditional laboratory models such as rodents, while numerous vertebrate species of ecological and economic relevance lack standardized anatomical references. This limitation hinders comparative studies and restricts the expansion of neuroscience toward non-traditional organisms.
The present project aims to standardize a methodological protocol for the development of a brain atlas in non-traditional vertebrates, with an initial focus on the freshwater fish Prochilodus magdalenae (bocachico) and future extension to domestic birds such as chickens, hens, and ducks. Bocachico represents the most advanced model in this study and is used as a reference species for protocol optimization.
Work has focused on standardizing post-euthanasia brain extraction conditions and determining the optimal section thickness for histological analyses compatible with Nissl staining. The protocol includes immediate fixation of the head in formalin following euthanasia, followed by a fixation period prior to brain dissection. Subsequently, the brain is fixed in 4% paraformaldehyde (PFA) and cryoprotected in 30% sucrose, then sectioned in a cryostat at 20 µm and initially processed using Nissl staining. The resulting sections allow visualization of brain cytoarchitecture and photographic documentation of major encephalic regions.
Standardization of this methodological protocol establishes the foundation for constructing reproducible neuroanatomical atlases in non-traditional species.
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