GluN1

Imaging neuronal morphology and activity pattern in developing cerebral cortex layer 4

Establishment of precise neuronal connectivity in the neocortex relies on activity-dependent circuit reorganization during postnatal development. In the mouse somatosensory cortex layer 4, barrels are arranged in one-to-one correspondence to whiskers on the face. Thalamocortical axon termini are clustered in the center of each barrel. The layer 4 spiny stellate neurons are located around the barrel edge, extend their dendrites primarily toward the barrel center, and make synapses with thalamocortical axons corresponding to a single whisker. These organized circuits are established during the first postnatal week through activity-dependent refinement processes. However, activity pattern regulating the circuit formation is still elusive. Using two-photon calcium imaging in living neonatal mice, we found that layer 4 neurons within the same barrel fire synchronously in the absence of peripheral stimulation, creating a ''patchwork'' pattern of spontaneous activity corresponding to the barrel map. We also found that disruption of GluN1, an obligatory subunit of the N-methyl-D-aspartate (NMDA) receptor, in a sparse population of layer 4 neurons reduced activity correlation between GluN1 knockout neuron pairs within a barrel. Our results provide evidence for the involvement of layer 4 neuron NMDA receptors in spatial organization of the spontaneous firing activity of layer 4 neurons in the neonatal barrel cortex. In the talk I will introduce our strategy to analyze the role of NMDA receptor-dependent correlated activity in the layer 4 circuit formation.

Acute effects of human monoclonal anti-GluN1 autoantibodies on NMDA-receptor channel function

Excitatory glycine GluN1/GluN3A receptors in the adult hippocampus

Human anti-GluN1 autoantibodies induce defective synaptic plasticity dependent on CaMKII and DAPK1 pathways as investigated by super-resolution microscopy

Pathogenic mutation GluN1-N650K in combination with GluN2A subunit changes kinetic parameters and conductance of NMDA receptors

Widespread expression and action of GluN1/GluN3A excitatory glycine receptors in the adult brain

Human anti-GluN1 autoantibodies affect CaMKII and DAPK1 pathway dependent synaptic plasticity in primary hippocampal mouse neurons

FENS Forum 2024

The role of disulfide bonds in GluN1 in the regulation of the early trafficking and functional properties of GluN1/GluN2 subtypes of NMDA receptors

FENS Forum 2024

The role of disulfide bonds in GluN1 in the regulation of the early trafficking and functional properties of GluN1/GluN3A subtypes of NMDA receptors

FENS Forum 2024