tissue clearing

Development and evolution of neuronal connectivity

In most animal species including humans, commissural axons connect neurons on the left and right side of the nervous system. In humans, abnormal axon midline crossing during development causes a whole range of neurological disorders ranging from congenital mirror movements, horizontal gaze palsy, scoliosis or binocular vision deficits. The mechanisms which guide axons across the CNS midline were thought to be evolutionary conserved but our recent results suggesting that they differ across vertebrates.  I will discuss the evolution of visual projection laterality during vertebrate evolution.  In most vertebrates, camera-style eyes contain retinal ganglion cell (RGC) neurons projecting to visual centers on both sides of the brain. However, in fish, RGCs are thought to only innervate the contralateral side. Using 3D imaging and tissue clearing we found that bilateral visual projections exist in non-teleost fishes. We also found that the developmental program specifying visual system laterality differs between fishes and mammals. We are currently using various strategies to discover genes controlling the development of visual projections. I will also present ongoing work using 3D imaging techniques to study the development of the visual system in human embryo.

CLEARING UP INCONTINENCE: DEVELOPING OPTICAL TISSUE CLEARING TECHNIQUES TO UNRAVEL THE 3D NEUROANATOMY OF BLADDER CONTROL IN THE HUMAN SPINAL CORD

FENS Forum 2026

CORRECTION OF AIR MICROSCOPE OBJECTIVES FOR USE IN SOLUTIONS FOR TISSUE CLEARING

FENS Forum 2026

RATDISCO: A TISSUE CLEARING PIPELINE FOR DETECTING STIMULUS-ACTIVATED NEURONS IN RAT MODELS OF DEVELOPMENTAL DISORDERS

FENS Forum 2026

RATDISCO, A NOVEL TISSUE CLEARING AND IMMUNOLABELLING PIPELINE FOR LARGE RAT BRAINS

FENS Forum 2026

ADVANCED TISSUE CLEARING APPROACHES FOR 3D PATHOLOGICAL BRAIN INVESTIGATION

FENS Forum 2026

3D TISSUE CLEARING IN NEUROSCIENCE : TO SEE IT BETTER, MAKE IT TRANSPARENT

FENS Forum 2026

Three-dimensional Visualization of Cerebral Blood Vessels and Neural Changes in Thick Ischemic Rat Brain Slices using Tissue Clearing

New Tissue clearing development

Detailed 3D architecture of adult human organs through new tissue clearing techniques

FENS Forum 2024

Combining electrophysiology, tissue clearing, and light sheet microscopy for an integrated approach towards brain circuit understanding

FENS Forum 2024