Hippocampal replay, the sequential reactivation of place cells, is postulated to be a mechanism central to memory consolidation. Commonly studied using highly repeated stereotyped behaviours (e.g. running back and forth on a linear track), we don’t understand how such memories are formed in more naturalistic settings, with a single or limited exposure, and the effect on subsequent replay. To further investigate this, we trained rats to run back on forth on two novel linear tracks each day, each track limited to a fixed but different number of laps. Following a post-behaviour sleep session, rats were re-exposed to both tracks again, but this time ran an equal amount of time on each track, followed by a second sleep session. This approach allowed us to (1) study sleep replay arising from differences in the amount of exposure to a novel environment [post first exposure], and (2) simultaneously examine how different degrees of familiarity influenced replay when the duration of the most recent exposure was similar [post re-exposure]. We found that as the rat ran additional laps along the novel track, the levels of sleep replay for that experience increased. However, when the rat was re-exposed to both tracks (for an equal amount of time), replay events were more prevalent for the track in which the rat had less experience on the initial exposure (less familiar). While theta sequences are postulated to be a central mechanism required for offline replay, we unexpectedly observed that the amount of awake replay during the most recent behavioural episode was the most accurate predictor of how much offline replay occurred during the subsequent sleep session. These results further our understanding of how experience shapes the encoding of different spatial trajectories, and the mechanisms modulating offline replay during the subsequent sleep memory consolidation.