This study delves into the complexities of glioblastoma multiforme (GBM), a highly aggressive brain cancer, with a particular focus on understanding the role of CD9, a transmembrane protein involved in tumor cell invasion, apoptosis, and resistance to chemotherapy. GBM presents major challenges due to its rapid progression and resistance to conventional treatments. Using immunocytochemistry, CD9 is used as a marker to identify distinct cell populations within GBM cultures. Simultaneously, the study investigates CD9 expression patterns by flow cytometry, providing valuable insights into its potential implications in GBM progression. The research is developed by characterizing different cell states in glioblastoma, especially those related to CD9. By elucidating the role of CD9 in various cellular contexts, the study seeks to contribute to the identification of this transmembrane protein as a potential therapeutic target. The emphasis lies in unraveling the dynamics of GBM and the specific involvement of CD9 in this malignancy. In conclusion, this study not only underscores the complexities of GBM, but also highlights the pivotal role played by CD9 in disease progression. Understanding the influence of CD9 on GBM cells lays the groundwork for potential targeted interventions, addressing the pressing need for more effective strategies against this disease. The integration of findings from both studies offers a comprehensive perspective, providing valuable insights into the intricate landscape of glioblastoma and the importance of CD9 in its pathogenesis.