Astrocytes, beyond their pivotal role as supporting cells, are recently considered to be dynamic modulators of neuronal activity and synaptic plasticity. This effect is mainly through their Ca2+ signaling cascades which result in a release of gliotransmitters. Glutamate as an important neurotransmitter which is crucial for many cognitive functions can modulate astrocytic Ca2+ signaling via their receptors. Considering that these processes have largely been studied in young animals, along with the association of aging with various diseases, this study aims to explore the age-dependent changes in astrocyte Ca2+ signaling through group I metabotropic glutamate receptors (mGluR), as well as investigate the impact of neuroinflammation on their activity. Using acute brain slices of C57BL/6 mice aged between postnatal days 7 and 26, we performed confocal Ca2+ imaging in the glomerular layer of the olfactory bulb. The results showed a significant decrease in the number of cells responding to agonist of group I metabotropic glutamate receptors, (R, S)-3,5-dihydroxyphenylglycine (DHPG), in addition to reduced response amplitudes, with increasing age. Moreover, response amplitudes of DHPG-evoked Ca2+ signals were significantly reduced in the presence of mGluR5 antagonists. However, experimental autoimmune encephalomyelitis (EAE)-induced neuroinflammation did not significantly alter astrocytic DHPG-induced responses in the glomerular layer. These findings support a developmental role for mGluR5 in modulating astrocyte activity, which may contribute to further understanding of the developmental process.