Methamphetamine (MA) is one of the most abused illicit drugs in the world. The abuse of this drug among adolescents is problematic due to the still developing physiological functions of the body, including the central nervous system (CNS). The hippocampus (HP) is a part of the limbic system that is associated with cognition, as well as the reward system, which is mediated by dopamine (DA). Disruption of this system is the basic mechanism underlying addiction and results in cognition deficiencies.In this work, we focused on the influence of MA on the development of the HP during postnatal days 11–20. Subsequently, after MA application, we studied learning and memory processes using the Morris Water Maze (MWM). After MWM, expression of the protein doublecortin (DCX), neurotransmitters (DA, GLU,5HT, NA and GABA) levels and glial fibrillary acidic protein (GFAP) were measured.Results of the present study indicate that MA significantly impairs HP neurogenesis when administered during the third postnatal week, as indicated by reduced expression of DCX, which correlates with increased levels of glial fibrillary acidic protein (GFAP), but these changes were not significantly reflected in learning abilities and memory formation nor in the levels of neurotransmitters.FundingThis research was supported by project 260648/SVV/2024 and the research program Cooperatio Neurosciences from Charles University.