In order to interrogate the connectivity and function of local hippocampal circuits, there is a strong need to develop viral gene transfer tools which selectively target distinct neuronal populations. Here we have developed a new recombinant-capsid AAV variants (rAAV) which can infect Dentate granule cells (DGC) with high efficiency and very good selectivity.We have used an AAV-peptide library in which each rAAV displays a random 9 amino acids peptide inserted into exposed loops of the AAV9 capsid. The library was injected in vivo by steretaxy in the mouse DG. After 3 rounds of in vivo selection, the detection of successful gene delivery was performed by identification of lead rAAVs via next generation sequencing (NGS). The selectivity of DGC infection by the variants library was finally determined by injection of the variants and benchmarks AAV serotypes in different off-target brain areas. Finally, we analyzed the infection pattern of the most selective rAAVs based on EYFP expression under the control of CMV (ubiquitous) or hSYN1 (neuronal specific) promoters. A quantitative cell populations analysis on brain sections of AI9 tomato mice injected with rAAV.hSyn.CRE-eGFP either in the hilus close to the DG or in CA3 area allowed to identify a rAAV-variant with high infection selectivity to DGCs and no infection of CA3 neurons or hilus neurons, as compared to the original AAV9 serotype.Our DG selective rAAV will be useful and flexible to combine gene transfer techniques and optogenetic manipulation of DGCs in hippocampus.