Animals integrate new information with their pre-existing memories to navigate their environment efficiently. Neuromodulation influences the balance between prior and current information, with β-adrenergic modulation playing a key role in memory and emotionally motivated processes (DOI: 10.1038/s41583-020-0360-9). We investigated the impact of central β-adrenergic activation on the renewal of a spatial appetitive learning task. Rats were trained to locate a low-probability reward in a T-Maze in context A, and the next day an extinction learning session took place in context B. After a day, they received intracerebral infusions of a beta-adrenergic receptor (B-AR) agonist, or vehicle, and after 30-min, were returned to context A which was now unrewarded, to test for renewal. Subsequently, the brains were collected, and fluorescence in situ hybridization for Homer 1a and Arc was conducted to characterize brain activation during the early and late stages of renewal. Interregional coordinated activity was then calculated, and graph-theory-based functional networks were generated and compared. Our results indicate that B-AR activation enhances renewal and alters patterns of brain activation, resulting in changes in network connectivity. B-AR activation during renewal triggered an initial increase in communication between hippocampus and subcortical, and a decrease between hippocampus and cortical structures. Later during testing, this changed into a state of low global coordinated activity that suggests modular activity superceded global activity. Our data indicate that β-AR activation shapes brain activation and drives network connectivity toward a state that favors the renewal of extinguished information.Supported by the Deutsche Forschungsgemeinschaft (SFB 1280/A04, project number: 316803389).