Hydrocephalus is associated with raised intracranial pressure (ICP) and has been implicated as the cause of endocrine dysfunction. Raised ICP results in distension of the third ventricle leading to compression of diencephalic structures and the hypothalamic-hypophyseal pathway. In this study, we aimed to assess the serum level of growth hormone and the histological changes in anterior pituitary gland of experimentally induced juvenile hydrocephalic rats. Hydrocephalus was induced in 3-week-old albino Wistar rats through intracisternal injection of sterile kaolin while age matched control rats received sham injections. After 4weeks, serum growth hormone assay, histology of the striatum (Hematoxylin and Eosin, H&E) and immunohistochemistry of the primary anti-growth hormone antibodies (ImageJ) in the anterior pituitary gland were assessed.The control rats gained weight at a faster rate than the hydrocephalic rats (p<0.0001). H&E sections of the anterior pituitary gland of hydrocephalic rats showed sparse cells with increased intercellular fibrous tissue and reduced eosinophilic intensity compared to controls. The mean density of the anterior pituitary gland somatotrophs of the hydrocephalic group was lower when compared to the control group (10.65±4.71 vs 15.66±5.46). The H&E sections of the striatum of the hydrocephalic rats showed enlarged ventricles with disruption of the ependymal lining. The mean serum growth hormone level of the hydrocephalic rats was lower than the controls (55±15.88 vs 57.65±19.85 ng/ml).Experimentally induced hydrocephalus in juvenile rats is associated with reduced somatotrophs density in the pituitary gland and subsequently reduced serum growth hormone levels.