Temporal lobe epilepsy (TLE) is characterized by spontaneous recurrent seizures poorly controlled by antiseizure medications in over 30% of patients. The current study aims to investigate the most epileptogenic areas in a TLE animal model using high-density EEG.Sprague Dawley male rats were injected intraperitoneally with pilocarpine to induce the status epilepticus (SE) terminated 30 minutes later by ketamine/diazepam. Two weeks later animals were implanted with epidural electrodes in the frontal cortex and depth electrodes in several temporal areas. Video-EEG recordings (24h, >3 days/week) were collected up to week 12 post-SE. Manual identification of ictal events was performed offline. Frequency domain (Welch method) and time-frequency domain (spectrogram) analyses were carried out. Experiments were performed in accordance to EU Directive 2010/63 and authorized by the Italian Ministry of Health.Our data show that 28% of focal-to-bilateral tonic clonic seizures (FBTCS) originate in the medial entorhinal cortex (mEC), while lower percentage begins in the ventral areas of the hippocampus, such as dentate gyrus (20%) and CA3 (17%). A preliminary power spectral density analysis shows that in the pre-ictal state the low frequency bands (delta and theta) prevail. In the ictal state a high frequency band (beta) stands out, while the post-ictal state is dominated exclusively by the lowest delta band.Our data show that high-density EEG provides a full characterization of the epileptic syndrome. In particular, we show here that both neocortical and limbic areas of the temporal lobe contribute to the pathology of TLE and should always be investigated together.