Stress is a physiological adaptive response towards physical and psychological stressors that can have profound impact on appetite. In addition to suppressing food intake, stress has also been shown to reduce reward seeking and cue-induced appetitive behaviours. Previous studies show that stress activates hindbrain nucleus of the solitary tract (NTS) neurons and NTS neurons have been implicated in stress-induced suppression of food intake. However, whether NTS neurons mediate the effects of stress on cue-induced appetitive behaviours are unclear. Using behavioural, pharmacogenetic and neural monitoring techniques, this study investigated the role of NTS neurons in stress-induced suppression of conditioned appetitive behaviour. Results showed that the stressor, foot shock suppressed cue-induced appetitive behaviour and chemogenetic inhibition of NTS neurons attenuated the inhibitory effects of foot shock on cue-induced appetitive behaviour. Subsequent analysis demonstrated that these effects are mediated in part, via a population of NTS neurons known as the A2 neurons as chemogenetic stimulation of NTS A2 neurons suppressed cue-induced appetitive behaviour. Fibre photometry experiments measuring midbrain dopamine or lateral hypothalamic neural activity revealed that stimulation of NTS A2 neurons suppressed midbrain dopamine, but not lateral hypothalamic neuron activity, during presentation of the food-predictive cue. This suggests a possible mechanism through which stress and NTS A2 neurons reduces cue-induced appetitive behaviours. Together, results from this study highlight the hindbrain NTS as a neural substrate mediating the effects of stress on cue-induced appetitive behaviours.