Neural stem cell (NSC) transplantation has emerged as a promising strategy for the therapy of several neurological diseases, however, the transplanted cells have shown a low survival rate and poor differentiation, mainly due to their inability to collect in the damaged area without leaking. Brain organoids are emerging as attractive tools for studying brain development and disease modeling and recently they acquired great attention for they potential use in regenerative medicine. Cerebral organoids are three-dimensional in vitro structures generated by exploiting the intrinsic self-aggregation capacity of NSCs, capable of recapitulating both functionally and structurally the brain complexity in vivo. We succeeded in generating murine cerebral organoids with specific CA3 hippocampal phenotype by adding a low concentration of the Wnt3a morphogen during the early stages of the protocol. This project aims to provide evidence of their potential use in regenerative medicine. We set up organoids transplantation by grafting immature organoids (14 days in vitro) obtained from embryonic hippocampal NSCs transduced with LV-GFP into ventral CA3 region of healthy adult rodents. We found organoid-derived cells expressing markers of neuronal differentiation suggesting their integration and maturation into the host tissue. Overall, these data highlight the potential use of hippocampal organoids as a tool for regenerative medicine purposes.